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Genetics in methanogens: transposon insertion mutagenesis of a Methanococcus maripaludis nifH gene.

We designed a transposon insertion mutagenesis system for Methanococcus species and used it to make mutations in and around a nifH gene in Methanococcus maripaludis. The transposon Mudpur was constructed with a gene for puromycin resistance that is expressed and selectable in Methanococcus species....

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Blank, C E, Kessler, P S, Leigh, J A
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1995
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177397/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7592322/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.20.5773-5777.1995
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