Expression and characterization of UDPGlc dehydrogenase (KfiD), which is encoded in the type-specific region 2 of the Escherichia coli K5 capsule genes.

Region 2 of the Escherichia coli K5 capsule gene cluster contains four genes (kfiA through -D) which encode proteins involved in the synthesis of the K5 polysaccharide. A DNA fragment containing kfiD was amplified by PCR and cloned into the gene fusion vector pGEX-2T to generate a GST-KfiD fusion pr...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Sieberth, V, Rigg, G P, Roberts, I S, Jann, K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177216/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7635844/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.15.4562-4565.1995
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