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Expression and characterization of UDPGlc dehydrogenase (KfiD), which is encoded in the type-specific region 2 of the Escherichia coli K5 capsule genes.

Region 2 of the Escherichia coli K5 capsule gene cluster contains four genes (kfiA through -D) which encode proteins involved in the synthesis of the K5 polysaccharide. A DNA fragment containing kfiD was amplified by PCR and cloned into the gene fusion vector pGEX-2T to generate a GST-KfiD fusion pr...

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Main Authors: Sieberth, V, Rigg, G P, Roberts, I S, Jann, K
Formato: Artigo
Idioma:Inglês
Publicado: 1995
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC177216/
https://ncbi.nlm.nih.gov/pubmed/7635844
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