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High-level expression of soluble recombinant RNase P protein from Escherichia coli.

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functi...

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Bibliografski detalji
Glavni autori: Rivera-León, R, Green, C J, Vold, B S
Format: Artigo
Jezik:Inglês
Izdano: 1995
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC176919/
https://ncbi.nlm.nih.gov/pubmed/7730292
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