Proton pumping and the internal pH of yeast cells, measured with pyranine introduced by electroporation.

The internal pH of yeast cells was determined by measuring the fluorescence changes of pyranine (8-hydroxy-1,3,6-pyrene-trisulfonic acid), which was introduced into the cells by electroporation. This may be a suitable procedure for the following reasons. (i) Only minor changes in the physiological s...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Peña, A, Ramírez, J, Rosas, G, Calahorra, M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1995
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC176697/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7860582/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.4.1017-1022.1995
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