Hyperproduction, purification, and mechanism of action of the cytotoxic enterotoxin produced by Aeromonas hydrophila.

A gene encoding the cytotoxic enterotoxin (Act) from Aeromonas hydrophila was hyperexpressed with the pET, pTRX, and pGEX vector systems. Maximum toxin yield was obtained with the pTRX vector. Approximately 40 to 60% of Act was in a soluble form with the pTRX and pET vector systems. The toxin protei...

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Bibliografiske detaljer

Udgivet i:	Infect Immun
Principais autores:	Ferguson, M R, Xu, X J, Houston, C W, Peterson, J W, Coppenhaver, D H, Popov, V L, Chopra, A K
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1997
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC175616/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9317040/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.65.10.4299-4308.1997
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