Hyperproduction, purification, and mechanism of action of the cytotoxic enterotoxin produced by Aeromonas hydrophila.

A gene encoding the cytotoxic enterotoxin (Act) from Aeromonas hydrophila was hyperexpressed with the pET, pTRX, and pGEX vector systems. Maximum toxin yield was obtained with the pTRX vector. Approximately 40 to 60% of Act was in a soluble form with the pTRX and pET vector systems. The toxin protei...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Ferguson, M R, Xu, X J, Houston, C W, Peterson, J W, Coppenhaver, D H, Popov, V L, Chopra, A K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC175616/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9317040/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.65.10.4299-4308.1997
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