Substrate-dependent mutant complementation to select fatty acid desaturase variants for metabolic engineering of plant seed oils

We demonstrate that naturally occurring C(14) and C(16)-specific acyl-acyl carrier protein (ACP) desaturases from plants can complement the unsaturated fatty acid (UFA) auxotrophy of an Escherichia coli fabA/fadR mutant. Under the same growth conditions, C(18)-specific Δ(9)-stearoyl (18:0)-ACP desat...

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Main Authors: Cahoon, Edgar B., Shanklin, John
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences 2000
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC17345/
https://ncbi.nlm.nih.gov/pubmed/11027301
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