Substrate-dependent mutant complementation to select fatty acid desaturase variants for metabolic engineering of plant seed oils
We demonstrate that naturally occurring C(14) and C(16)-specific acyl-acyl carrier protein (ACP) desaturases from plants can complement the unsaturated fatty acid (UFA) auxotrophy of an Escherichia coli fabA/fadR mutant. Under the same growth conditions, C(18)-specific Δ(9)-stearoyl (18:0)-ACP desat...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC17345/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11027301/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.210276297 |
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