Construction and use of integration plasmids to generate site-specific mutations in the Actinomyces viscosus T14V chromosome.
Stable transformants of Actinomyces viscosus T14V carrying heterologous DNA were obtained with the aid of integration plasmids. These plasmids contained a kanamycin resistance (Kmr) gene flanked by A. viscosus T14V genomic DNA, including parts of the type 1 structural fimbrial subunit gene (fimP) on...
Guardat en:
| Publicat a: | Infect Immun |
|---|---|
| Autor principal: | |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1995
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC173398/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7622214/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.63.8.2924-2930.1995 |
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