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Rapid nucleic acid assay for detection of bacteria with tetM-mediated tetracycline resistance.

A novel nucleic acid assay has been developed to screen bacterial populations for the presence of the tetM structural gene. The method involves the specific hybridization of several synthetic oligonucleotides to the gene in a crude bacterial lysate solution. As few as 1.5 x 10(4) CFU can be detected...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Antimicrob Agents Chemother
Egile Nagusiak: Sanchez-Pescador, R, Running, J A, Stempien, M M, Urdea, M S
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology (ASM) 1989
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC172761/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2511803/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.33.10.1813
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