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Rapid nucleic acid assay for detection of bacteria with tetM-mediated tetracycline resistance.

A novel nucleic acid assay has been developed to screen bacterial populations for the presence of the tetM structural gene. The method involves the specific hybridization of several synthetic oligonucleotides to the gene in a crude bacterial lysate solution. As few as 1.5 x 10(4) CFU can be detected...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Antimicrob Agents Chemother
Autori principali: Sanchez-Pescador, R, Running, J A, Stempien, M M, Urdea, M S
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 1989
Soggetti:
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC172761/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2511803/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.33.10.1813
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