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On the tryptophan residue of smooth muscle myosin that responds to binding of nucleotide

Initially, we asked which (of 10) smooth muscle myosin head residues responds to MgADP or MgATP binding with enhanced fluorescence emission (Trp-441 and Trp-512 were leading candidates)? To decide, we prepared sham-mutated smooth muscle heavy meromyosin (HMM), W441F HMM, and W512F HMM. On adding MgA...

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Detalhes bibliográficos
Main Authors: Onishi, Hirofumi, Konishi, Kaoru, Fujiwara, Keigi, Hayakawa, Kazuyoshi, Tanokura, Masaru, Martinez, Hugo M., Morales, Manuel F.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC17178/
https://ncbi.nlm.nih.gov/pubmed/11016961
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