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Gene transfer and expression in human neutrophils. The phox homology domain of p47(phox )translocates to the plasma membrane but not to the membrane of mature phagosomes

BACKGROUND: Neutrophils are non-dividing cells with poor survival after isolation. Consequently, exogenous gene expression in neutrophils is challenging. We report here the transfection of genes and expression of active proteins in human primary peripheral neutrophils using nucleofection. RESULTS: E...

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Detalhes bibliográficos
Main Authors: Johnson, Jennifer L, Ellis, Beverly A, Munafo, Daniela B, Brzezinska, Agnieszka A, Catz, Sergio D
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1712351/
https://ncbi.nlm.nih.gov/pubmed/17150107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2172-7-28
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