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Gene transfer and expression in human neutrophils. The phox homology domain of p47(phox )translocates to the plasma membrane but not to the membrane of mature phagosomes

BACKGROUND: Neutrophils are non-dividing cells with poor survival after isolation. Consequently, exogenous gene expression in neutrophils is challenging. We report here the transfection of genes and expression of active proteins in human primary peripheral neutrophils using nucleofection. RESULTS: E...

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Autori principali: Johnson, Jennifer L, Ellis, Beverly A, Munafo, Daniela B, Brzezinska, Agnieszka A, Catz, Sergio D
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1712351/
https://ncbi.nlm.nih.gov/pubmed/17150107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2172-7-28
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