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Improved procedure for crystallization of Clostridium botulinum type A toxic complexes.

A modified protocol for the preparation of botulinum type A toxic crystals is decribed. The essential change was the substitution of chromatography on diethylaminoethyl-Sephadex for the step in which toxin was precipitated with ethanol. Colored materials and nucleic acids were removed by the chromat...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Sugiyama, H, Moberg, L J, Messer, S L
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1977
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC170797/
https://ncbi.nlm.nih.gov/pubmed/405926
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