Improved procedure for crystallization of Clostridium botulinum type A toxic complexes.
A modified protocol for the preparation of botulinum type A toxic crystals is decribed. The essential change was the substitution of chromatography on diethylaminoethyl-Sephadex for the step in which toxin was precipitated with ethanol. Colored materials and nucleic acids were removed by the chromat...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1977
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC170797/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/405926/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.33.4.963-966.1977 |
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