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Improved procedure for crystallization of Clostridium botulinum type A toxic complexes.

A modified protocol for the preparation of botulinum type A toxic crystals is decribed. The essential change was the substitution of chromatography on diethylaminoethyl-Sephadex for the step in which toxin was precipitated with ethanol. Colored materials and nucleic acids were removed by the chromat...

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Detalhes bibliográficos
Main Authors: Sugiyama, H, Moberg, L J, Messer, S L
Formato: Artigo
Idioma:Inglês
Publicado em: 1977
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC170797/
https://ncbi.nlm.nih.gov/pubmed/405926
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