A simplified method for Ca2+ flux measurement on isolated human B cells that uses flow cytometry.
A method for Ca2+ flux measurement on isolated human peripheral B cells that uses flow cytometry is described. B cells were isolated by anti-CD19 magnetic bead sorting, and Ca2+ flux was measured with the fluo-3 reagent on a standard single-laser flow cytometer. The response of B-cell stimulation by...
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| Publié dans: | Clin Diagn Lab Immunol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1997
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC170478/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9008284/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/cdli.4.1.70-74.1997 |
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