A simplified method for Ca2+ flux measurement on isolated human B cells that uses flow cytometry.

A method for Ca2+ flux measurement on isolated human peripheral B cells that uses flow cytometry is described. B cells were isolated by anti-CD19 magnetic bead sorting, and Ca2+ flux was measured with the fluo-3 reagent on a standard single-laser flow cytometer. The response of B-cell stimulation by...

Popoln opis

Bibliografske podrobnosti

izdano v:	Clin Diagn Lab Immunol
Principais autores:	Gergely, L, Cook, L, Agnello, V
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1997
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC170478/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9008284/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/cdli.4.1.70-74.1997
Oznake:	Označite Brez oznak, prvi označite!