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Method to protect a targeted amino acid residue during random mutagenesis

To generate a random mutant library that is free from mutation at a particular amino acid residue, we replace the codon of interest with a detachable, short DNA sequence containing a BsaXI recognition site. After PCR mutagenesis, this sequence is removed and intramolecular ligation of the sequences...

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Bibliografiske detaljer
Main Authors: Umeno, Daisuke, Hiraga, Kaori, Arnold, Frances H.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC169983/
https://ncbi.nlm.nih.gov/pubmed/12907743
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