Method to protect a targeted amino acid residue during random mutagenesis

To generate a random mutant library that is free from mutation at a particular amino acid residue, we replace the codon of interest with a detachable, short DNA sequence containing a BsaXI recognition site. After PCR mutagenesis, this sequence is removed and intramolecular ligation of the sequences...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Umeno, Daisuke, Hiraga, Kaori, Arnold, Frances H.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2003
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169983/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12907743/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng091
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