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Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA–protein conjugates

An assay for very sensitive antigen detection is described which takes advantage of the self- assembly capabilities of semi-synthetic conjugates of DNA and proteins. The general scheme of this assay is similar to a two-sided (sandwich) enzyme-linked immunoassay (ELISA); however, covalent single-stra...

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Библиографические подробности
Опубликовано в::Nucleic Acids Res
Главные авторы: Niemeyer, Christof M., Wacker, Ron, Adler, Michael
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2003
Предметы:
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169982/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12907742/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng090
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