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Parallel competition analysis of Saccharomyces cerevisiae strains differing by a single base using polymerase colonies

We describe a strategy to analyze the impact of single nucleotide mutations on protein function. Our method utilizes a combination of yeast functional complementation, growth competition of mutant pools and polyacrylamide gel immobilized PCR. A system was constructed in which the yeast PGK1 gene was...

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Udgivet i:Nucleic Acids Res
Principais autores: Merritt, Joshua, DiTonno, Jason R., Mitra, Robi D., Church, George M., Edwards, Jeremy S.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2003
Fag:
NAR Methods Online
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169973/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12888536/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng084
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