A simple two-step, ‘hit and fix’ method to generate subtle mutations in BACs using short denatured PCR fragments

The bacteriophage lambda recombination system has proven to be a valuable tool for engineering bacterial artificial chromosomes (BAC). Due to its high efficiency, subtle alterations in the BACs can be generated using oligonucleotides as targeting vectors. Since no selection marker is used, recombina...

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書目詳細資料

發表在:	Nucleic Acids Res
Principais autores:	Yang, Yongping, Sharan, Shyam K.
格式:	Artigo
語言:	Inglês
出版:	Oxford University Press 2003
主題:	NAR Methods Online
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169966/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12888532/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng080
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