A simple two-step, ‘hit and fix’ method to generate subtle mutations in BACs using short denatured PCR fragments

The bacteriophage lambda recombination system has proven to be a valuable tool for engineering bacterial artificial chromosomes (BAC). Due to its high efficiency, subtle alterations in the BACs can be generated using oligonucleotides as targeting vectors. Since no selection marker is used, recombina...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Yang, Yongping, Sharan, Shyam K.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2003
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169966/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12888532/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng080
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