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Comparison of a PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Assay to Pulsed-Field Gel Electrophoresis To Determine the Effect of Repeated Subculture and Prolonged Storage on RFLP Patterns of Shiga Toxin-Producing Escherichia coli O157:H7

In this study, we compared a recently developed PCR-restriction fragment length polymorphism (PCR-RFLP) assay with pulsed-field gel electrophoresis (PFGE) using three different Shiga toxin-producing Escherichia coli (STEC) strains to understand whether repeated subculture in vitro and prolonged stor...

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Autori principali: Shima, Kensuke, Wu, Yuluo, Sugimoto, Norihiko, Asakura, Masahiro, Nishimura, Kazuhiko, Yamasaki, Shinji
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1698327/
https://ncbi.nlm.nih.gov/pubmed/16971640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00717-06
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