Method for reproducible large-volume production and purification of Rauscher murine leukemia virus.

Rauscher murine leukemia virus was produced in roller-bottle cultures of chronically infected JLS-V9 cells. Virus from this culture fluid was concentrated and purified by two semi-isopycnic bandings in sucrose gradients. Virus material obtained from young, nonconfluent cultures (early-harvest virus)...

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Dades bibliogràfiques

Publicat a:	Appl Environ Microbiol
Autors principals:	Johnson, R W, Perry, A, Robinson, O R, Shibley, G P
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1976
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169745/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/63267/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.31.2.182-188.1976
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