Method for reproducible large-volume production and purification of Rauscher murine leukemia virus.
Rauscher murine leukemia virus was produced in roller-bottle cultures of chronically infected JLS-V9 cells. Virus from this culture fluid was concentrated and purified by two semi-isopycnic bandings in sucrose gradients. Virus material obtained from young, nonconfluent cultures (early-harvest virus)...
Salvato in:
| Pubblicato in: | Appl Environ Microbiol |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
1976
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169745/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/63267/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.31.2.182-188.1976 |
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