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In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner-family transposable element Himar1

We present here a method for in vivo transposon mutagenesis of a methanogenic archaeon, Methanosarcina acetivorans C2A, which because of its independence from host-specific factors may have broad application among many microorganisms. Because there are no known Methanosarcina transposons we modified...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Zhang, J. K., Pritchett, M. A., Lampe, D. J., Robertson, H. M., Metcalf, W. W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2000
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC16922/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10920201/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.160272597
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