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In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner-family transposable element Himar1

We present here a method for in vivo transposon mutagenesis of a methanogenic archaeon, Methanosarcina acetivorans C2A, which because of its independence from host-specific factors may have broad application among many microorganisms. Because there are no known Methanosarcina transposons we modified...

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Detalhes bibliográficos
Main Authors: Zhang, J. K., Pritchett, M. A., Lampe, D. J., Robertson, H. M., Metcalf, W. W.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC16922/
https://ncbi.nlm.nih.gov/pubmed/10920201
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