Alteration of Chain Length Substrate Specificity of Aeromonas caviae R-Enantiomer-Specific Enoyl-Coenzyme A Hydratase through Site-Directed Mutagenesis

Aeromonas caviae R-specific enoyl-coenzyme A (enoyl-CoA) hydratase (PhaJ(Ac)) is capable of providing (R)-3-hydroxyacyl-CoA with a chain length of four to six carbon atoms from the fatty acid β-oxidation pathway for polyhydroxyalkanoate (PHA) synthesis. In this study, amino acid substitutions were i...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Tsuge, Takeharu, Hisano, Tamao, Taguchi, Seiichi, Doi, Yoshiharu
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2003
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169158/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902277/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4830-4836.2003
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