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Enhanced Accumulation and Changed Monomer Composition in Polyhydroxyalkanoate (PHA) Copolyester by In Vitro Evolution of Aeromonas caviae PHA Synthase

By in vitro evolution experiment, we have first succeeded in acquiring higher active mutants of a synthase that is a key enzyme essential for bacterial synthesis of biodegradable polyester, polyhydroxyalkanoate (PHA). Aeromonas caviae FA440 synthase, termed PhaC(Ac), was chosen as a good target for...

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Bibliografiske detaljer
Main Authors: Kichise, Tomoyasu, Taguchi, Seiichi, Doi, Yoshiharu
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2002
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC127549/
https://ncbi.nlm.nih.gov/pubmed/11976116
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.5.2411-2419.2002
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