Dual Labeling of Pseudomonas putida with Fluorescent Proteins for In Situ Monitoring of Conjugal Transfer of the TOL Plasmid

We describe here a dual-labeling technique involving the green fluorescent protein (GFP) and the red fluorescent protein (DsRed) for in situ monitoring of horizontal gene transfer via conjugation. A GFPmut3b-tagged derivative of narrow-host-range TOL plasmid (pWWO) was delivered to Pseudomonas putid...

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Autores principales: Nancharaiah, Y. Venkata, Wattiau, Pierre, Wuertz, Stefan, Bathe, Stephan, Mohan, S. Venkata, Wilderer, Peter A., Hausner, Martina
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2003
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC169129/
https://ncbi.nlm.nih.gov/pubmed/12902279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4846-4852.2003
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