Dual Labeling of Pseudomonas putida with Fluorescent Proteins for In Situ Monitoring of Conjugal Transfer of the TOL Plasmid

We describe here a dual-labeling technique involving the green fluorescent protein (GFP) and the red fluorescent protein (DsRed) for in situ monitoring of horizontal gene transfer via conjugation. A GFPmut3b-tagged derivative of narrow-host-range TOL plasmid (pWWO) was delivered to Pseudomonas putid...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Nancharaiah, Y. Venkata, Wattiau, Pierre, Wuertz, Stefan, Bathe, Stephan, Mohan, S. Venkata, Wilderer, Peter A., Hausner, Martina
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Methods
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169129/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902279/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4846-4852.2003
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