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Identification and Application of Plasmids Suitable for Transfer of Foreign DNA to Members of the Genus Gordonia
Gene transfer systems for Gordonia polyisoprenivorans strains VH2 and Y2K based on electroporation and conjugation, respectively, were established. Several parameters were optimized, resulting in transformation efficiencies of >4 × 10(5) CFU/μg of plasmid DNA. In contrast to most previously descr...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology
2003
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC169124/ https://ncbi.nlm.nih.gov/pubmed/12902293 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4971-4974.2003 |
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