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Identification and Application of Plasmids Suitable for Transfer of Foreign DNA to Members of the Genus Gordonia

Gene transfer systems for Gordonia polyisoprenivorans strains VH2 and Y2K based on electroporation and conjugation, respectively, were established. Several parameters were optimized, resulting in transformation efficiencies of >4 × 10(5) CFU/μg of plasmid DNA. In contrast to most previously descr...

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Detalhes bibliográficos
Main Authors: Arenskötter, Matthias, Baumeister, Dirk, Kalscheuer, Rainer, Steinbüchel, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC169124/
https://ncbi.nlm.nih.gov/pubmed/12902293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4971-4974.2003
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