Is the In Situ Accessibility of the 16S rRNA of Escherichia coli for Cy3-Labeled Oligonucleotide Probes Predicted by a Three-Dimensional Structure Model of the 30S Ribosomal Subunit?

Systematic studies on the hybridization of fluorescently labeled, rRNA-targeted oligonucleotides have shown strong variations in in situ accessibility. Reliable predictions of target site accessibility would contribute to more-rational design of probes for the identification of individual microbial...

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Bibliographic Details
Main Authors: Behrens, Sebastian, Fuchs, Bernhard M., Mueller, Florian, Amann, Rudolf
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2003
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC169109/
https://ncbi.nlm.nih.gov/pubmed/12902289
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4935-4941.2003
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