Real-Time PCR for Simultaneous Detection and Quantification of Borrelia burgdorferi in Field-Collected Ixodes scapularis Ticks from the Northeastern United States

The density of spirochetes in field-collected or experimentally infected ticks is estimated mainly by assays based on microscopy. In this study, a real-time quantitative PCR (qPCR) protocol targeting the Borrelia burgdorferi-specific recA gene was adapted for use with a Lightcycler for rapid detecti...

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Autori principali: Wang, Guiqing, Liveris, Dionysios, Brei, Brandon, Wu, Hongyan, Falco, Richard C., Fish, Durland, Schwartz, Ira
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2003
Soggetti:
Public Health Microbiology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC169074/
https://ncbi.nlm.nih.gov/pubmed/12902243
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4561-4565.2003
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