Real-Time PCR for Simultaneous Detection and Quantification of Borrelia burgdorferi in Field-Collected Ixodes scapularis Ticks from the Northeastern United States

The density of spirochetes in field-collected or experimentally infected ticks is estimated mainly by assays based on microscopy. In this study, a real-time quantitative PCR (qPCR) protocol targeting the Borrelia burgdorferi-specific recA gene was adapted for use with a Lightcycler for rapid detecti...

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Bibliografische Detailangaben

Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Wang, Guiqing, Liveris, Dionysios, Brei, Brandon, Wu, Hongyan, Falco, Richard C., Fish, Durland, Schwartz, Ira
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2003
Schlagworte:	Public Health Microbiology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169074/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902243/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4561-4565.2003
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