Immunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples.

A method to detect viable Cryptosporidium parvum oocysts was developed. Polyclonal immunoglobulin G against C. parvum oocyst and sporozoite surface antigens was purified from rabbit immune serum, biotinylated, and bound to streptoavidin-coated magnetic particles. C. parvum oocysts were captured by a...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Deng, M Q, Cliver, D O, Mariam, T W
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168611/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9251200/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.8.3134-3138.1997
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