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Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR.

Current methods for detection of Cryptosporidium parvum oocysts in water are time-consuming and difficult. We have developed a reverse transcription (RT)-PCR which can detect the presence of a single viable oocyst spiked into concentrated environmental water samples. The test is based on the detecti...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Stinear, T, Matusan, A, Hines, K, Sandery, M
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168136/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8795230/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.9.3385-3390.1996
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