PCR-based approach for detection of novel Bacillus thuringiensis cry genes.
A two-step strategy, named exclusive PCR or E-PCR, has been developed to overcome the main limitation of PCR, which is the detection of already-known sequences only. This strategy allows the ability to detect and further clone and sequence genes for which no specific primers are available and in whi...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9251188/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.8.2997-3002.1997 |
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