PCR-based approach for detection of novel Bacillus thuringiensis cry genes.

A two-step strategy, named exclusive PCR or E-PCR, has been developed to overcome the main limitation of PCR, which is the detection of already-known sequences only. This strategy allows the ability to detect and further clone and sequence genes for which no specific primers are available and in whi...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Juárez-Pérez, V M, Ferrandis, M D, Frutos, R
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1997
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9251188/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.8.2997-3002.1997
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