Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids.

Plasmids were constructed in which DNA damage-inducible promoters recA, uvrA, and alkA from Escherichia coli were fused to the Vibrio fischeri luxCDABE operon. Introduction of these plasmids into E. coli allowed the detection of a dose-dependent response to DNA-damaging agents, such as mitomycin and...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Vollmer, A C, Belkin, S, Smulski, D R, Van Dyk, T K, LaRossa, R A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9212407/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.7.2566-2571.1997
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