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Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph.

We report the fist genetic transformation system, shuttle vectors, and integrative vectors for the thermotolerant, methylotrophic bacterium Bacillus methanolicus. By using a polyethylene glycol-mediated transformation procedure, we have successfully transformed B. methanolicus with both integrative...

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Détails bibliographiques
Auteurs principaux: Cue, D, Lam, H, Dillingham, R L, Hanson, R S, Flickinger, M C
Format: Artigo
Langue:Inglês
Publié: 1997
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC168436/
https://ncbi.nlm.nih.gov/pubmed/9097439
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