Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph.

We report the fist genetic transformation system, shuttle vectors, and integrative vectors for the thermotolerant, methylotrophic bacterium Bacillus methanolicus. By using a polyethylene glycol-mediated transformation procedure, we have successfully transformed B. methanolicus with both integrative...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Cue, D, Lam, H, Dillingham, R L, Hanson, R S, Flickinger, M C
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1997
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168436/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9097439/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.4.1406-1420.1997
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