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Direct ribosome isolation from soil to extract bacterial rRNA for community analysis.

A simple method that combines an adapted ribosome isolation method and a common RNA extraction step has been developed for selective recovery of intact rRNA from natural microbial communities in soil. After mechanical cell lysis, ribosomes are separated by centrifugation steps, avoiding massive humi...

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Bibliografski detalji
Glavni autori: Felske, A, Engelen, B, Nübel, U, Backhaus, H
Format: Artigo
Jezik:Inglês
Izdano: 1996
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC168238/
https://ncbi.nlm.nih.gov/pubmed/8900007
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