A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization.

This article reports the development of a method to purify and concentrate intact virions from oyster extracts to volume and quality compatible with viral genomic nucleic acid amplification by reverse transcriptase PCR (RT-PCR) and confirmation by oligonucleotide probe hybridization. Fifty-gram oyst...

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Bibliografische Detailangaben

Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Jaykus, L A, De Leon, R, Sobsey, M D
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1996
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167985/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8787405/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.6.2074-2080.1996
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