A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization.
This article reports the development of a method to purify and concentrate intact virions from oyster extracts to volume and quality compatible with viral genomic nucleic acid amplification by reverse transcriptase PCR (RT-PCR) and confirmation by oligonucleotide probe hybridization. Fifty-gram oyst...
Spremljeno u:
| Izdano u: | Appl Environ Microbiol |
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| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1996
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167985/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8787405/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.6.2074-2080.1996 |
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