Highly efficient expression of fish growth hormone by Escherichia coli cells.

A PCR product encoding the mature segment of fish pregrowth hormone (pre-GH) was inserted into an Escherichia coli expression vector, pET, in which the ori site was replaced by that of pUC19. The yield of recombinant GH (rGH) was as high as 44 to 47% of total protein. This rGH was immunoreactive to...

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Bibliografiska uppgifter

I publikationen:	Appl Environ Microbiol
Huvudupphov:	Tsai, H J, Lin, K L, Kuo, J C, Chen, S W
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	American Society for Microbiology (ASM) 1995
Ämnen:	Research Article
Länkar:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167720/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8526527/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.11.4116-4119.1995
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