Highly efficient expression of fish growth hormone by Escherichia coli cells.

A PCR product encoding the mature segment of fish pregrowth hormone (pre-GH) was inserted into an Escherichia coli expression vector, pET, in which the ori site was replaced by that of pUC19. The yield of recombinant GH (rGH) was as high as 44 to 47% of total protein. This rGH was immunoreactive to...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Tsai, H J, Lin, K L, Kuo, J C, Chen, S W
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167720/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8526527/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.11.4116-4119.1995
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